Understanding Peptide Purity: What 99% Does and Doesn’t Tell You
A purity figure is a chromatographic ratio, not a statement about everything in the vial. What the number measures, what it leaves out and how to read it.
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A purity figure is a chromatographic ratio, not a statement about everything in the vial. What the number measures, what it leaves out and how to read it.
HPLC separates a peptide from its impurities on a column and reports the result as a chromatogram. Here is how it works and how to read one on a COA.
A supplier grading its own product is a conflict of interest, not a quality system. What independent testing adds, what it cannot fix, how to read it.
Purity tells you how much of a sample is one thing. Mass spectrometry tells you what that thing is. Here is how the identity check works.
A vial can be 99% pure and 75% peptide by weight at the same time. Counter-ions, bound water and how amino-acid analysis resolves the difference.
How a peptide is built one amino acid at a time on a resin bead, why Merrifield's method won a Nobel Prize, and where impurities enter the process.
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